Category Archives: Data analysis

Whole-cell patch clamp, part 3: Limitations of quantitative whole-cell voltage clamp

Before I first dived into experimental neuroscience, I imagined whole-cell voltage clamp recordings to be the holy grail of precision. Directly listening to the currents that take place inside of a living neuron! How beautiful and precise, compared to poor-resolution techniques like fMRI or … Continue reading

Posted in Data analysis, electrophysiology, Neuronal activity, zebrafish | Tagged , , | 3 Comments

Spike detection competition

The main drawback of functional calcium imaging is its slow dynamics. This is not only due to limited frame rates, but also due to calcium dynamics, which are a slow transient readout of fast spiking activity. A perfect algorithm would infer the … Continue reading

Posted in Calcium Imaging, Data analysis, machine learning | Tagged , , | 1 Comment

Neuroscience on Youtube

Recently, I’ve been to the Basel ICON conference, where the recent Nobel laureate Eric Betzig gave an impressive talk on microscopy techniques (including lattice light sheet, SIM and expansion microscopy). Some days ago, I found a similar talk by Eric … Continue reading

Posted in Data analysis, Microscopy, Network analysis | Tagged , | Leave a comment

Spatial visualization of temporal components for neuronal activity imaging

The standard analysis workflow for neuronal activity imaging based on calcium signals is to 1) draw ROIs around putative neurons, 2) extract the average fluorescence time trace of this ROI, 3) work with this timetrace for subsequent analysis (principal components, … Continue reading

Posted in Calcium Imaging, Data analysis, Imaging, Neuronal activity | Tagged , | Leave a comment

Beyond correlation analysis: Dynamic causal modeling (DCM)

I was surprised to find a method like DCM in Olav Stetter’s list (link) for neural network methods (even as a so-called ‘standard method’), because it differs from those I discussed before. I will now describe why I don’t think … Continue reading

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Beyond correlation analysis: Transfer entropy

When reading through the first informative web pages on transfer entropy, it turns out how closely its concept is related to mutual information, and even closer to incremental mutual information; and, although it’s based on a totally different approach, it tries to … Continue reading

Posted in Data analysis, Network analysis | 1 Comment

Beyond correlation analysis: mutual information

Last time, I mentioned a website which gives an overview of methods to analyze neuronal (and other) networks. Let’s have a closer look. Here’s a list of the methods: Cross-correlation (the standard method) Mutual Information Incremental Mutual Information Granger Causality … Continue reading

Posted in Data analysis, Network analysis | 3 Comments