Category Archives: Microscopy

Can two-photon scanning be too fast?

The following back-of-the-envelope calculations do not lead to any useful result, but you might be interesting in reading through them if you want to get a better understanding of what happens during two-photon excitation microscopy. The basic idea of two-photon microscopy … Continue reading

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All-optical entirely passive laser scanning with MHz rates

Is it possible to let a laser beam scan over an angle without moving any mechanical parts to deflect the beam? It is. One strategy is to use a very short-pulsed laser beam: A short pulse width means a finite … Continue reading

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Whole-cell patch clamp, part 2: Line-frequency pick-up via the perfusion system

With the experience of more than one year of patching (although you might say that this is not a lot), I’m now used to problems that I can solve after some time, but without being able to tell what the problem has … Continue reading

Posted in electrophysiology, Microscopy | Tagged | 1 Comment

Matlab code for control of a resonant scanning microscope

For control of resonant scanning 2P microscopes, my host lab uses a software that I have written in Matlab. Due to some coincidences, the software is based on Scanimage 4.2, a version developed few years ago for an interface with a Thorlabs scope and Thorlabs … Continue reading

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Preamplifier bandwidth & two ways of counting photons

For two-photon point scanning microscopy, the excitation laser is typically pulsing at a repetition rate of 80 MHz, that is one pulse each 12.5 ns. To avoid aliasing, it was suggested to synchronize the sampling clock to laser pulses. For this, it is important … Continue reading

Posted in Calcium Imaging, Imaging, Microscopy | Tagged , , , | 2 Comments

Whole-cell patch clamp, part 1: introductory reading

Ever since I my interested in neuroscience become more serious, I was fascinated by the patch clamp technique, especially applied for the whole cell. Calcium imaging or multi-channel electrophysiology (recent review) is the way to go in order to get an idea what … Continue reading

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Neuroscience on Youtube

Recently, I’ve been to the Basel ICON conference, where the recent Nobel laureate Eric Betzig gave an impressive talk on microscopy techniques (including lattice light sheet, SIM and expansion microscopy). Some days ago, I found a similar talk by Eric … Continue reading

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