Tag Archives: Microscopy

Whole-cell patch clamp, part 4: look and feel

In previous blog posts, I have been discussing some aspects of whole-cell patch clamp recordings ([1], [2], [3], [4]). Today, I will show some instructive videos that I recorded during experiments. I’m hoping that they will convey the look and feel … Continue reading

Posted in Calcium Imaging, electrophysiology, Imaging, Microscopy, Neuronal activity, zebrafish | Tagged , , , | 1 Comment

Alvarez lenses and other strangely shaped optical elements

In typical microscopes, lenses or mirrors are moved forth and back to change the position of their focus. Tunable lenses like the electro-tunable lens or the TAG lens, on the other hand, are deformed by an external force and thereby … Continue reading

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Open access 3D electron microscopy datasets of brains

One of the coolest technical developments in neuroscience during the last decade has been driven by 3D electron microscopy (3D EM). This allowed to cut large junks of small brains (or small junks of big brains) into 8-50 nm thick … Continue reading

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Springtime for two-photon microscopy

Today, the fields and forests around Basel are full of flowers that try to disseminate their pollen. Fixed pollen are, apart from sub-diffraction beads and the convallaria rhizome, one of the most commonly used test/reference samples for fluorescence microscopy. This … Continue reading

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Can two-photon scanning be too fast?

The following back-of-the-envelope calculations do not lead to any useful result, but you might be interesting in reading through them if you want to get a better understanding of what happens during two-photon excitation microscopy. The basic idea of two-photon microscopy … Continue reading

Posted in Imaging, Microscopy | Tagged , , , , | 8 Comments

All-optical entirely passive laser scanning with MHz rates

Is it possible to let a laser beam scan over an angle without moving any mechanical parts to deflect the beam? It is. One strategy is to use a very short-pulsed laser beam: A short pulse width means a finite … Continue reading

Posted in Calcium Imaging, Imaging, Microscopy | Tagged , , | 1 Comment

Matlab code for control of a resonant scanning microscope

For control of resonant scanning 2P microscopes, my host lab uses a software that I have written in Matlab. Due to some coincidences, the software is based on Scanimage 4.2, a version developed few years ago for an interface with a Thorlabs scope and Thorlabs … Continue reading

Posted in Imaging, Microscopy | Tagged , , | 1 Comment