Tag Archives: zebrafish

Whole-cell patch clamp, part 4: look and feel

In previous blog posts, I have been discussing some aspects of whole-cell patch clamp recordings ([1], [2], [3], [4]). Today, I will show some instructive videos that I recorded during experiments. I’m hoping that they will convey the look and feel … Continue reading

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Precise synaptic balance of excitation and inhibition

The main paper of my PhD just got published: Rupprecht and Friedrich, Precise Synaptic Balance in the Zebrafish Homolog of Olfactory Cortex, Neuron (2018). (PDF) You might like it if you are also interested in Classical balanced networks Things you … Continue reading

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Open access 3D electron microscopy datasets of brains

One of the coolest technical developments in neuroscience during the last decade has been driven by 3D electron microscopy (3D EM). This allowed to cut large junks of small brains (or small junks of big brains) into 8-50 nm thick … Continue reading

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Whole-cell patch clamp, part 3: Limitations of quantitative whole-cell voltage clamp

Before I first dived into experimental neuroscience, I imagined whole-cell voltage clamp recordings to be the holy grail of precision. Directly listening to the currents that take place inside of a living neuron! How beautiful and precise, compared to poor-resolution techniques like fMRI or … Continue reading

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The crow as an animal model for neuroscience

Close to my apartment in the outskirts of Basel, green fields and some small woods lie basically in front of my house door. This is also where some flocks of crows gather around, partly searching the fields for food, partly … Continue reading

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Whole-cell patch clamp, part 1: introductory reading

Ever since I my interested in neuroscience become more serious, I was fascinated by the patch clamp technique, especially applied for the whole cell. Calcium imaging or multi-channel electrophysiology (recent review) is the way to go in order to get an idea what … Continue reading

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The larval zebrafish, and the adult zebrafish

Zebrafish are often used as a model organism for in vivo brain imaging, because they are transparent. Or at least that is what many people think who do not work with zebrafish. In reality, most people use zebrafish larvae for in vivo … Continue reading

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